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KMID : 0861020110260030015
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Korea Journal of Herbology
2011 Volume.26 No. 3 p.15 ~ p.21
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PCR Analysis for the Discrimination of Leonuri Herba Medicine on the Basis of Chloroplast DNA Sequence Comparison in Six Lamiaceae Species
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Lee Jae-Woong
Kim Young-Hwa
Choi Go-Ya
Ko Byoung-Seob
Kim Young-Sun
Chae Sung-Wook
Lee Hye-Won
Oh Seung-Eun
Park Sang-Un
Lee Mi-Young
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Abstract
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Objectives:The application of polymerase chain reaction (PCR) for the discrimination of the herbal medicine Leonuri Herba (Leonurus japonicus) was evaluated by the comparison of the DNA sequence with Lamiaceae herbal medicine.
Method:Genetic analysis showed that phylogenetic tree and comparing sequences through the DNA analysis of rbcL (ribulose-1, 5-bisphosphatecarboxylase) region and trnL-F (tRNA-Leu, trnL-trnF intergeni cspacer, and tRNA-Phe) region of chloroplast DNA from six Lamiaceae sold in market. And we developed IMCF and IMCR primers in order to distinction Leonuri Herba in six Lamiaceae using rbcL and trnL-F sequences.
Results: Genetic analysis showed that six Lamiaceae showed individual group on phylogenetic tree. PCR amplification product of Leonuri Herba and another five Lamiaceae were developed for amplification of a 281 bp sequence and the specific PCR amplification of a 460 bp sequence that was exclusive to Leonuri Herba was designed using IMCF and IMCR primers.
Conclusion: PCR analysis based on the chloroplast DNA sequences allows the discrimination of Leonuri Herba-based medicine.
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KEYWORD
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PCR analysis, Chloroplast DNA, Leonurus japonicus, Lamiaceae
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